Part:BBa_K1104201:Experience

Introduction

TrxCp (Part:BBa_K1104201) is an OxyR-induced promoter, and was used as sensor to ROS (Reactive Oxygen Species).

TrxC promoter +E0840 (Part:BBa_K1104241)

We designed the device: TrxC promoter +E0840 (Part:BBa_K1104241) includes TrxC promoter (Part:BBa_K1104241) and Part:BBa_E0840, induced TrxC promoter by different concentrations of H₂O₂, and identified the strength.

In this device, after TrxCp (Part:BBa_K1104201) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced TrxC promoter by different concentrations of H₂O₂, and measured the expression of GFP, and identified the strength.

We characterized TrxCp by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. It shows the protocols we used for promoter testing. More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

Results

This is the relative promoter strength of BBa_K1104201 under different H₂0₂ concentrations tested using BBa_K11042041 (BBa_K1104201 +E0840) and found that there is a trend where the higher the H₂0₂ concentration, the higher the promoter expression:

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

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